Isolation, Identification and Screening of Chitinase Producing Aspergillus Niger from Soil
Keywords:chitinase, chitin, Aspergillus niger, isolation, screening
Chitin is one of the most abundant biopolymers widely distributed in the marine and terrestrial environments. Chitinase enzyme has received increased attention due to its wide range of biotechnological applications.In the present study an attempt was made to isolate organisms that would synthesize chitinaseChitinase may convert the biomass containing chitin into useful components (depolymerized).
This project aimed to isolation and identification of chitinase enzyme producing Fungi such as Aspergillus niger. In order to isolate chitinase from fungi, the possibility to obtain this enzyme from higher organisms such as animals, plants or insects are less. Since production of chitinase from higher organisms is a tedious process and also its purification is challenging.
Aspergillus niger was found to be a good chitinase producer among the five fungi isolated from different soil samples Punjab(India). Maximum production of chitinase was obtained when using 2% of Shrimp-shell powder as a sole carbon source in the fermentation medium. The high level of chitinase production was observed in the culture medium.
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