Gene Editing Experiment Using the CRISPR-Cas9 System
DOI:
https://doi.org/10.26821/IJSRC.10.6.2022.100608Keywords:
CRISPR, CRISPR-Cas9, E. coli, gene editing, DNA, RNA, gRNA, crRNAAbstract
An experimental report on using the CRISPR-Cas9 system to edit the gene and change the trait of E. coli
References
Ishino, Y., et al., Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product. Journal of bacteriology, 1987. 169(12): p. 5429-5433.
Barrangou, R., et al., CRISPR provides acquired resistance against viruses in prokaryotes. Science, 2007. 315(5819): p. 1709-1712.
Cong, L., et al., Multiplex genome engineering using CRISPR/Cas systems. Science, 2013. 339(6121): p. 819-823.
San Filippo, J., P. Sung, and H. Klein, Mechanism of eukaryotic homologous recombination. Annu. Rev. Biochem., 2008. 77: p. 229-257.
Schwank, G., et al., Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients. Cell stem cell, 2013. 13(6): p. 653-658.
Hoban, M.D., et al., CRISPR/Cas9-mediated correction of the sickle mutation in human CD34+ cells. Molecular Therapy, 2016. 24(9): p. 1561-1569.
Park, C.-Y., et al., Functional correction of large factor VIII gene chromosomal inversions in hemophilia A patient-derived iPSCs using CRISPR-Cas9. Cell stem cell, 2015. 17(2): p. 213-220.
Jiang, W., et al., RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nature biotechnology, 2013. 31(3): p. 233-239.
Sanders, E.R., Aseptic laboratory techniques: plating methods. JoVE (Journal of Visualized Experiments), 2012(63): p. e3064.
Markings, S. The Chemical Composition of Nutrient Agar. 2018; Available from: https://sciencing.com/ chemical-composition-nutrient-agar-14135.html.
Hinshaw, H. and W. Feldman. Streptomycin in Treatment of Clinical Tuberculosis: a Preliminary Report. in Proceedings of Staff Meetings of the Mayo Clinic. 1945.
Jawetz, E. and M. Sonne, Penicillin-streptomycin treatment of enterococcal endocarditis: a re- evaluation. New England Journal of Medicine, 1966. 274(13): p. 710-715.
Hall, W.H. and W.W. Spink, In vitro Sensitivity of Brucella to Streptomycin: Development of Resistance During Streptomycin Treatment. Proceedings of the Society for Experimental Biology and Medicine, 1947. 64(4): p. 403-406.
Luzzatto, L., D. Apirion, and D. Schlessinger, Mechanism of action of streptomycin in E. coli: interruption of the ribosome cycle at the initiation of protein synthesis. Proceedings of the National Academy of Sciences of the United States of America, 1968. 60(3): p. 873.
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Copyright (c) 2022 Grace Yongeun Song
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